CRISPRdirect is a web server for CRISPR-Cas9 guide RNA design. The web server supports 350+ species including animals, plants and fungi.
All services are freely available to all users and no login required.
Naito Y. et al. (2015) CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites. Bioinformatics, 31, 1120-1123. http://doi.org/10.1093/bioinformatics/btu743