<% _.each(terms.slice(0, 3), function(term, idx) { %>

<%= term._highlightResult.name.value %>

<% }) %>
Tools
Discover the best online tools to assist you in research

PCRduplicates

Are you the creator of this tool?

Suggested by: Roy Granit

Description

PCRduplicates is a tool that allows to estimate PCR duplication rate from high-throughput sequencing data

PCR amplification is an important step in the preparation of DNA sequencing libraries prior to high-throughput sequencing. Existing computational methods for analysis of read duplicates assume that all read duplicates arise due to PCR amplification. However, a high rate of read duplicates is observed in deep sequencing experiments or experiments such as RNA-seq. We present a computational method that exploits the heterozygosity in diploid genomes to estimate the PCR duplication rate accounting for read duplicates that are not due to PCR amplification.

Discussion

Ask a question or post a review

12  upvotes
0 collections
41 views
Show more

Similar/Related tools