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Suggested by: Roy Granit


PCRduplicates is a tool that allows to estimate PCR duplication rate from high-throughput sequencing data

PCR amplification is an important step in the preparation of DNA sequencing libraries prior to high-throughput sequencing. Existing computational methods for analysis of read duplicates assume that all read duplicates arise due to PCR amplification. However, a high rate of read duplicates is observed in deep sequencing experiments or experiments such as RNA-seq. We present a computational method that exploits the heterozygosity in diploid genomes to estimate the PCR duplication rate accounting for read duplicates that are not due to PCR amplification.


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